Tutorials

Downloads

• Latest demo version of CYANA 3.98.13 for Linux and MacOS (20.01.2021)
  The demo version has the full functionality of the program for the protein sequences that are used in the example calculations.
• On certain Mac systems, you must execute the command 'xattr -r -d com.apple.quarantine cyana-3.98.13' after unpacking to allow execution of the programs.

INCLAN Tutorials

• Writing and using INCLAN macros
• Using INCLAN variables
• Using INCLAN control statements

CYANA Tutorials

• Defining non-standard residues

CYANA example calculations

• Basic structure calculation starting from given restraints
• Structure calculation using manually assigned NOESY peak lists
• Structure calculation with automated NOESY assignment
• Homodimer structure calculation with automated NOESY assignment
• ENORA and multi-state structure calculations
• Identification of key NOEs
• Determination of protein state populations
• Determination of the protein number of states

Courses

EMBO Practical Course: Structure, dynamics and function of biomacromolecules by NMR 2019

Garching bei München, 26 July - 2 August 2019 (course homepage)

• Structure calculation and automated NOESY assignment with CYANA
• Automated resonance assignment with FLYA

Input file formats

• Residue library file (.lib)
• Sequence file (.seq)
• Distance restraint file (.upl, .lol)
• Torsion angle restraint file (.aco)
• Residual dipolar coupling restraint file (.rdc)
• Pseudocontact shift restraint file (.pcs)
• DG Cartesian coordinate file (.cor)
• PDB coordinate file (.pdb)
• Torsion angle file (.ang)
• XEASY chemical shift list file (.prot)
• BMRB chemical shift list file (.bmrb)
• XEASY peak list file (.peaks)
• NMRView peak list file (.xpk)