Tutorials

Downloads

• Latest demo version of CYANA 3.98.13 for Linux and MacOS (23.11.2021)
  The demo version has the full functionality of the program for the protein sequences that are used in the example calculations.
• On certain Mac systems, you must execute the command 'xattr -r -d com.apple.quarantine cyana-3.98.13' after unpacking CYANA to allow execution of the programs.
• Results of all CYANA 3.98.13 demo calculations (93 MB).

INCLAN Tutorials

• Writing and using INCLAN macros
• Using INCLAN variables
• Using INCLAN control statements

CYANA Tutorials

• Defining non-standard residues

CYANA example calculations

• Basic structure calculation starting from given restraints
• Structure calculation using manually assigned NOESY peak lists
• Structure calculation with automated NOESY assignment
• Homodimer structure calculation with automated NOESY assignment
• ENORA and multi-state structure calculations
• Identification of key NOEs
• Determination of the protein state populations
• Determination of the protein number of states
• NOESY peaklist preparation for the exact NOE pipeline (multiple mixing times)

Courses

Biomolecular NMR: Advanced tools PhD course

Gothenburg, 27 September - 8 October 2021 (course homepage)

• Automated resonance assignment with FLYA
• Structure calculation and automated NOESY assignment with CYANA

Input file formats

• Residue library file (.lib)
• Sequence file (.seq)
• Distance restraint file (.upl, .lol)
• Torsion angle restraint file (.aco)
• Residual dipolar coupling restraint file (.rdc)
• Pseudocontact shift restraint file (.pcs)
• DG Cartesian coordinate file (.cor)
• PDB coordinate file (.pdb)
• Torsion angle file (.ang)
• XEASY chemical shift list file (.prot)
• BMRB chemical shift list file (.bmrb)
• XEASY peak list file (.peaks)
• NMRView peak list file (.xpk)